The Effect of Suplementation of Sambiloto (Andrographis paniculata) Extract Through Drinking Water on Total Erythrocytes and Total Leucocytes of Broiler

  • Muhammad Mirandy Pratama Sirat Study Program of Animal Feed Nutrition and Technology, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung http://orcid.org/0000-0003-0088-5112
  • Madi Hartono Study Program of Animal Feed Nutrition and Technology, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung
  • Purnama Edy Santosa Study Program of Animal Husbandry, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung
  • Ratna Ermawati Study Program of Animal Husbandry, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung http://orcid.org/0000-0001-6529-5231
  • Teo Achmad Fauzi Study Program of Animal Feed Nutrition and Technology, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung
  • Nur Aini Study Program of Animal Feed Nutrition and Technology, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung
  • Fina Arzakiyah Study Program of Animal Husbandry, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung
  • Imam Widodo Study Program of Animal Husbandry, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung
  • Teo Achmad Fauzan Study Program of Animal Husbandry, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung
Keywords: Andrographis paniculata; Broilers; Drinking water; Total erythrocytes; Total leukocytes

Abstract

The aim of this study was to determine the effect and the best dose of Sambiloto (Andrographis paniculata) extract supplementation on total erythrocytes and total leukocytes in broilers. The study was conducted for 6 months, May – October 2021. Maintenance and treatment of broilers for 30 days was carried out at the Karang Anyar Farm broiler cage unit in Karang Anyar Village, Jati Agung District, South Lampung Regency, Lampung Province. The experimental study used a completely randomized design with five treatments. Each treatment consisted of 25 broilers of the Cobb CP 707 strain which were divided into 5 cages, so a total of 125 broilers in 25 cages. Sambiloto (Andrographis paniculata) as an immunomodulator was added to drinking water with different doses according to broiler body weight, namely drinking water without a liquid extract of Sambiloto (Andrographis paniculata) (P0); drinking water with Sambiloto liquid extract 0.05 cc/kg BW (3 mg/kg BW) (P1); 0.1 cc/kg BW (6 mg/kg BW) (P2); 0.2 cc/kg BW (12 mg/kg BW) (P3); 0.4 cc/kg BW (24 mg/kg BW) (P4). Total of 25 blood samples from 30-day-old broiler were taken through the brachial vein using a 3 mL syringe and stored in a 5 mL EDTA tube. Calculation of total erythrocytes and total leukocytes at the Palembang Health Laboratory and then the data were analyzed descriptively. The highest total erythrocyte in broilers was P0, the lowest was P4, the mean total erythrocyte was within normal limits for all treatments. The highest broiler leukocyte total was P2, the lowest was P3, the average total leukocyte for all treatments was above the normal range. The active compound in Sambiloto (Andrographis paniculata) is andrographolide which can act as an immunostimulant for specific and non-specific immune functions through NK cells, macrophages, and cytokine induction. The flavonoid content in sambiloto will also send intracellular signals to cell receptors to increase its activity when immune system activity is reduced which can increase the production of IL-2 thereby increasing lymphocyte proliferation, increasing the proliferation and differentiation of B and NK cells. The conclusion was that supplementation of Sambiloto (Andrographis paniculata) extract through drinking water can maintain the number of erythrocytes within normal limits and increase the number of leukocytes as an immune response after vaccination with the best supplementation dose in drinking water of 6 mg/kg BW which is equivalent to a volume of 0.1 cc/liquid extract. kg BB broiler.

Author Biography

Muhammad Mirandy Pratama Sirat, Study Program of Animal Feed Nutrition and Technology, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung

Veterinary Medicine; Large Animal Reproduction

References

Azlan, A., L. Younis, N.H. Mahmud, and N.A. Dardiri. 2013. Mechanism of action of Andrographis paniculata as anti atherosclerotic agent. European International J. Sci. Tech. 2(2): 1-6

Badan Pusat Statistik. 2021. Statistik Indonesia Statistical Yearbook of Indonesia 2021. No. Katalog 1101001. Badan Pusat Statistik. Jakarta

Cahyaningsih, U, D. Iswantini dan Iskandar. 2007. Pemanfaatan Tanaman Sambiloto (Andrographis paniculata) sebagai Substitusi Obat Anti Coccidia dan Anti Peradangan untuk Menanggulangi Diare Berdarah pada Ayam akibat Infeksi Eimeria tenella. Skripsi. Institut Pertanian Bogor. Bogor.

Desmawati. 2013. Sistem Hematologi dan Imunologi. In Media. Jakarta.

Illah, Z.A., R.D. Ratnani, Suwardiyono, I. Hartati. 2014. Ekstraksi hidrotopi dengan magnetic stirer untuk mendapatkan senyawa androghapholide dari tanaman sambiloto (Andrographis paniculata). Momentum, 10(1): 38-42.

Londok, J., K.G. Wiryawan, W. Manalu, Sumiati. 2018. Profil Hematologi Ayam Pedaging yang Diberi Ransum Mengandung Asam Laurat dan Pinang Yaki Sebagai Sumber Antioksidan Alami. Jurnal Veteriner, 19(2): 222-229. DOI: 10.19087/jveteriner.2018.19.2.222

Kumar, A., J. Dora, A. Singh, and R. Tripathi. 2012. A review on King of Bitter (Kalmegh). Int. J. Res. Pharma. Chem. 2:116-124.

Khumairoh, Tjandrakirana, W. Budijastuti. 2013. Pengaruh Pemberian Filtrat Daun Sambiloto terhadap Jumlah Leukosit Darah Tikus Putih yang Terpapar Benzena. LenteraBio. 2(1): 1-5.

Maharani, E.A. and G. Noviar. 2018. Imunohematologi dan Bank Darah (Badan Pengembangan dan Pemberdayaan Sumber Daya Manusia Kesehatan, Jakarta.

Mohamed, E.A.A., O.H.A. Ali, E.E.H. Malik, I.A. Yousif. 2012. Effect of season and dietary protein level on some haematological parameters and blood biochemical composition of three broiler strains. Intl. J. Poult. Sci., 11(12): 787-793.

Nugroho, A.E., M. Andrie, N.K. Warditiani, E. Siswanto, S. Pramono, and E. Lukitaningsih. 2012. Antidiabetic and antihiperlipidemic effect of Andrographis paniculata (Burm. f.) Nees and andrographolide in high-fructose-fat-fed rats. Indian J. Pharmacol. 44(3): 377-381

Permatasari, D.F. 2019. Pengaruh Pemberian Ekstrak Daun Sambiloto (Andrographis paniculata ness.) terhadap Jumlah Eritrosit pada Tikus (Rattus norvegicus) yang diinduksi Gentamisin Dosis Toksik. Skripsi. Universitas Airlangga. Surabaya

Praseno, K. 2005. Respon eritrosit terhadap perlakuan mikromineral Cu, Fe, dan Zn pada ayam (Gallus gallus domesticus). J. Indo Tropical Animal Agriculture, 30(3) : 179 -185

Purnomo, D., Sugiharo, and Isroli. 2015. Total leukosit dan diferensial leukosit darah ayam broiler akibat penggunaan tepung onggok fermentasi rhizopus oryzae pada ransum. J. Ilmu-Ilmu Peternakan. 25(3) : 59 – 68.

Ratnani, D.R., I. Hartati, and L. Kurniasari. 2012. Potensi Andrographolide dari Sambiloto (Andrographis paniculata Ness) Melalui Proses Ekstraksi Hidrotropi. Momentum, 8(1): 6-10

Sa’adah, S. 2018. Sistem Peredaran Darah Manusia. UIN Sunan Gunung Djati. Bandung.

Sapitri, H.M., P.E. Santosa, S. Suharyati, and M.M.P. Sirat. 2021. Total Eritrosit dan Leukosit Broiler Jantan setelah Pemberian Jintan Hitam (Nigella sativa) sebagai Imunomodulator dalam Air Minum. Jurnal Riset dan Inovasi Peternakan, 5(1): 22-29.

Sasmito, E. 2017. Imunomodulator Bahan Alami. Andi Offset. Jakarta.

Sheeja, K., P.K. Shihab, and G. Kuttan. 2006. Antioxidant and Anti-Inflammatory Activities of the Plant Andrographis paniculata Nees. Immunopharmacology and Immunotoxicology, 28(1): 129-140

Sheeja K, G. Kuttan. 2007. Modulation of natural killer activity, antibody dependent cellular toxicity, and antibody dependent complement mediated cytotoxicity by andrographolide in normal and ehrlich ascites carcinoma bearing mice. Integr Cancer Ther. 6: 66-73.

Siswanto. 2017. Diktat Fisiologi Veteriner 1 Darah dan Cairan Tubuh. Fakultas Kedokteran Hewan Universitas Udayana. Bali.

Smith, J.B. and S. Mangkoewidjojo. 1988. Pemeliharaan, Pembiakan dan Penggunaan Hewan Percobaan di Daerah Tropis. UI-Press. Jakarta.

Sukmayadi, A.E., S.A. Sumiwi, M.I. Barliana, A.D. Aryanti. 2014. Aktivitas imunomodulator ekstrak etanol daun Tempuyung (Sonchus arvensis Linn.). Indonesian Journal of Pharmaceutical Science and Technology. 1(2): 65-72.

Tizzard, I.R. 2000. Immunology: An Introduction. 6th Ed. Saunders. New York

Wahju. 2004. Ilmu Nutrisi Unggas. Yogyakarta: Universitas Gajah Mada Press.

Wang W., J. Wang, S.F. Dong, C.H. Liu, P. Italiani, S.H. Sun, J. Xu, D. Boraschi, S.P. Ma, D. Qu. 2010. Immunomodulatory activity of andrographolicle on macrofage activation and specific antibody response. Acta pharmacol Sin. 31(2): 191-201. DOI: 10.1038/aps.2009.205.

Weiss, D.J. dan K.J. Wardrop. 2010. Schalm’s Veterinary Hematology. 6th Edition Wiley Blackwell. Iowa.

Yuniwarti, E.Y.W. 2015. Profil darah ayam broiler setelah vaksinasi AI dan pemberian berbagai kadar VCO. Buletin Anatomi dan Fisiologi, 23(1) : 38-46

Published
2022-04-01
How to Cite
Sirat, Muhammad Mirandy, Madi Hartono, Purnama Santosa, Ratna Ermawati, Teo Fauzi, Nur Aini, Fina Arzakiyah, Imam Widodo, and Teo Fauzan. 2022. “The Effect of Suplementation of Sambiloto (Andrographis Paniculata) Extract Through Drinking Water on Total Erythrocytes and Total Leucocytes of Broiler”. Jurnal Riset Dan Inovasi Peternakan (Journal of Research and Innovation of Animals) 6 (1), 74-82. http://jrip.fp.unila.ac.id/index.php/JRIP/article/view/190.
Issue
Vol 6 No 1 (2022): Jurnal Riset dan Inovasi Peternakan: Februari 2022
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Articles

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